1 00:00:00,000 --> 00:00:03,000 Stephen Hurry: Here’s the nucleus with this nuclear membrane around it. 2 00:00:05,000 --> 00:00:10,000 The only structure that can be seen are these dark objects here. 3 00:00:12,000 --> 00:00:16,000 These are called nucleoli. They have no function in cell division. 4 00:00:17,000 --> 00:00:22,000 Notice that the rest of the nucleus contains apparently nothing special, 5 00:00:23,000 --> 00:00:27,000 but chemical activity is going on here and it’s at this stage… 6 00:00:28,000 --> 00:00:33,000 ..in the division process, that the DNA is duplicated. 7 00:00:34,000 --> 00:00:36,000 This stage is called Interphase. 8 00:00:38,000 --> 00:00:44,000 What happens next? Well, inside the nucleus the chromosomes become apparent. 9 00:00:45,000 --> 00:00:49,000 The nucleoli vanish and the nuclear membrane bursts apart. 10 00:00:50,000 --> 00:00:54,000 Watch it here. We’ve made a jump so this is rather late in the process. 11 00:00:55,000 --> 00:01:00,000 You can see the chromosomes, now they become free in the cytoplasm. 12 00:01:10,000 --> 00:01:13,000 Here we are, here’s the same cell again. 13 00:01:15,000 --> 00:01:20,000 Here are the chromosomes and you can see on this one, and on this one… 14 00:01:21,000 --> 00:01:26,000 ..over here that the chromosomes are double along their whole length. 15 00:01:27,000 --> 00:01:32,000 This is a consequence of the duplication that took place at the Interphase stage. 16 00:01:32,000 --> 00:01:35,000 The doubleness of the chromosomes extends down their whole length… 17 00:01:36,000 --> 00:01:41,000 ..except for one special region, here is one such region… 18 00:01:41,000 --> 00:01:44,000 ..and here on this chromosome another. 19 00:01:45,000 --> 00:01:49,000 These special regions are called centromeres and they are in fact… 20 00:01:50,000 --> 00:01:56,000 ..holding the double chromosome together. This stage is called Prophase. 21 00:01:58,000 --> 00:02:03,000 What happens next is that the chromosomes move together in the… 22 00:02:03,000 --> 00:02:07,000 ..centre of the cell and will line up across this equator. 23 00:02:09,000 --> 00:02:16,000 The centromere divides, setting the doubled chromosomes free in the cell. 24 00:02:16,000 --> 00:02:18,000 Let’s see it happen. 25 00:02:19,000 --> 00:02:23,000 Here you are, the chromosomes are unravelling, becoming shorter and thicker, 26 00:02:29,000 --> 00:02:32,000 and are now beginning to line up. 27 00:02:39,000 --> 00:02:43,000 There. Now here’s the same cell again and there’s the equator… 28 00:02:44,000 --> 00:02:47,000 ..along which the cell, the chromosomes are aligned. 29 00:02:48,000 --> 00:02:53,000 The centromeres divide, though you can’t see that on the photograph, 30 00:02:54,000 --> 00:02:57,000 and this stage is called Metaphase. 31 00:02:58,000 --> 00:03:01,000 The next stages happen quite quickly. 32 00:03:02,000 --> 00:03:05,000 The now separated parts of the chromosome move, 33 00:03:06,000 --> 00:03:09,000 one to this end, and one to that end of the cell. 34 00:03:10,000 --> 00:03:15,000 At the same time, a new cell wall will become visible forming across the equator. 35 00:03:17,000 --> 00:03:21,000 And also around the chromosomes at the ends of the… 36 00:03:21,000 --> 00:03:25,000 ..daughter cells a new nuclear membrane will appear. 37 00:03:26,000 --> 00:03:30,000 Now you can see this if you watch not only this cell but this one… 38 00:03:30,000 --> 00:03:34,000 ..and this one, all three at the same time, for in these two cells… 39 00:03:35,000 --> 00:03:40,000 ..the process is slightly in advance of the process in this big cell. 40 00:03:41,000 --> 00:03:43,000 So let’s see that happen. 41 00:03:45,000 --> 00:03:50,000 Now the chromosomes are separating , some of them… 42 00:03:51,000 --> 00:03:54,000 ..fairly reluctantly as if the chromosomes were sticking together. 43 00:03:58,000 --> 00:04:03,000 There they go and the last one. An arrow will appear in a moment… 44 00:04:04,000 --> 00:04:11,000 ..simply to show the direction of movement. There it is. 45 00:04:12,000 --> 00:04:15,000 Now you can begin to see the beginnings of the cell wall… 46 00:04:16,000 --> 00:04:23,000 ..forming across the middle, growing inwards from the edges. 47 00:04:29,000 --> 00:04:32,000 The cell wall’s very distinct now. 48 00:04:35,000 --> 00:04:38,000 Here are the last stages in the division of the cell. 49 00:04:39,000 --> 00:04:42,000 In this cell the chromosomes have retreated to the ends of the daughter cells. 50 00:04:43,000 --> 00:04:46,000 A new cell wall is forming between them and… 51 00:04:47,000 --> 00:04:51,000 ..in these two cells the final stages can be seen. 52 00:04:52,000 --> 00:04:55,000 Notice that the structure of the chromosomes is being lost and… 53 00:04:56,000 --> 00:04:59,000 ..in this lower nucleus, even less of the chromosomes can be seen. 54 00:05:00,000 --> 00:05:03,000 And around each, a new nuclear membrane will be forming. 55 00:05:04,000 --> 00:05:08,000 Daughter cells, each now with their own nucleus and separated… 56 00:05:09,000 --> 00:05:13,000 ..by a cell wall, so that we have two cells where we started with one.