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Description
This programme is one of a series of six which concentrate on crucial biochemical techniques; their practice and applications presented in a studio based laboratory setting. The speakers in the pro...gramme demonstrate various uses of radio-isotopes in investigating the dynamic aspects of cell biochemistry. The principles and practice of liquid scintillation counting are demonstrated and then used in the performance of an actual experiment - the comparison of the rate of protein synthesis in the liver of starved and fed rats.
This programme is one of a series of six which concentrate on crucial biochemical techniques; their practice and applications presented in a studio based laboratory setting. The speakers in the pro...gramme demonstrate various uses of radio-isotopes in investigating the dynamic aspects of cell biochemistry. The principles and practice of liquid scintillation counting are demonstrated and then used in the performance of an actual experiment - the comparison of the rate of protein synthesis in the liver of starved and fed rats.
| Item code: | S2-1; 04 |
|---|---|
| First transmission date: | 09-09-1972 |
| Published: | 1972 |
| Rights Statement: | |
| Restrictions on use: | |
| Duration: | 00:25:00 |
| + Show more... | |
| Producer: | Roger Jones |
| Contributors: | Stephen Rose; Brian Tiplady |
| Publisher: | BBC Open University |
| Keyword(s): | Biochemical techniques; Cell biochemistry; Liquid scintillation counting; Practice and applications; Protein synthesis; Radio-isotope uses; Rat livers |
| Footage description: | Stephen Rose introduces the programme. It will examine the rise of radio isotopes in the study of the dynamics of living matter. Rose discusses the rises of radio isotopes: 1. As markers to show where molecules have gone in the living organisms. 2. To show what compounds have been formed in the organism. Brian Tiplady demonstrates the Geiger counter on some radio-active samples. Tiplady explains the precautions necessary for handling radio-active materials. The samples tested with the Geiger counter are the isotopes P32, C14, H3. Tiplady explains why the three isotopes show different results under the counter Tiplady explains how radiation can be counted using the principle that radiation can excite certain materials to give off visible light. He demonstrates on an apparatus which has this capability. A flash of light is seen on the screen for every gamma particle which hits it. Tiplady demonstrates how a sample is prepared for radiation counting experiments. Tiplady with a scintillation counter. He explains how the machine works and demonstrates. A frequency histogram is plotted on a display screen (radiation energy in KEV/number of counts). Tiplady compares the frequency histogram for the three isotope samples which have been put through the scintillation counter. (P32,C14,H3) Tiplady introduces Stephen Rose's experiment. Stephen Rose introduces his examination of the rate of protein amino acids in a rat. (The experiment is described in detail in the broadcast notes) Rose begins the demonstration. He removes the liver from a freshly killed rat for analysis. The sample is frozen in liquid nitrogen to stop enzyme reaction. Rose lists the items which will be tested for in the experiment and then carries out the rest of the experiment. |
| Master spool number: | 6LT/70489 |
| Production number: | 00521_2131 |
| Videofinder number: | 2818 |
| Available to public: | no |
