Available online
Description
This programme deals with a Day School Experiment which students on the Physiology of Cells and Organisms course have to carry out during the year. The experiment is concerned with the study of the... acclimation of flies maintained at three different temperatures. The students must dissect the flies, measure metabolic rate using a Warburg manometer, record the results and process them. In addition, students must also handle data from oxygen electrode measurements on mitochondria from the insect's flight muscle. The programme sets out to acquaint the student with some of the features of the apparatus they will be using, the experimental conditions, the elementary dissection technique and the method of recording results. The programme also covers an elementary explanation of the principles of an oxygen electrode.
This programme deals with a Day School Experiment which students on the Physiology of Cells and Organisms course have to carry out during the year. The experiment is concerned with the study of the... acclimation of flies maintained at three different temperatures. The students must dissect the flies, measure metabolic rate using a Warburg manometer, record the results and process them. In addition, students must also handle data from oxygen electrode measurements on mitochondria from the insect's flight muscle. The programme sets out to acquaint the student with some of the features of the apparatus they will be using, the experimental conditions, the elementary dissection technique and the method of recording results. The programme also covers an elementary explanation of the principles of an oxygen electrode.
| Module code and title: | S321, Physiology of cells and organisms |
|---|---|
| Item code: | S321; 14 |
| First transmission date: | 08-09-1974 |
| Published: | 1974 |
| Rights Statement: | |
| Restrictions on use: | |
| Duration: | 00:24:00 |
| + Show more... | |
| Producer: | John Groom |
| Contributors: | Richard Holmes; Mike Tribe |
| Publisher: | BBC Open University |
| Keyword(s): | Acclimation of flies; Dissection; Mitochondria; Oxygen electrode; Results; Warburg manometer |
| Footage description: | The programme deals with the Day School experiments on the effect of acclimation of flies, at three different temperatures on their oxygen uptake. Richard Holmes introduces the programme. Richard Holmes with three containers which hold fly populations acclimatised at 30°c, 20°c, 10°c respectively. He captures a blow fly from one of the containers and demonstrates a method of placing it into a gelatin capsule for the experiment. Mike Tribe with a Warburg respirometer. He points out its components and explains how it works. Tribe places a prepared fly into the massometer as he talks. Richard Holmes shows students how to fill in the data sheets for this experiment. Mike Tribe takes readings from the Warburg respirometer and passes them to Richard Holmes who records them. Richard Holmes records further readings after a 5 minute interval. Richard Holmes and Mike Tribe give students a few hints on finishing this part of the experiment. Richard Holmes examines techniques for measuring tissue oxygen uptake in blowflies. He gives some tips on dissecting flies and demonstrates on a large model. Richard Holmes examines the problem of determining oxygen uptake at the sub-cellular level. Mike Tribe with electron micrographs of blowfly muscle fibres. He points out the characteristics of insect muscle tissue and then explains how the sample is prepared for measuring oxygen uptake at the subcellular level. Tribe explains how an oxygen electrode (the measuring device) works. Richard Holmes with a large cross section diagram of an oxygen electrode. He also explains how it works. Mike Tribe demonstrates the oxygen electrode and obtains a curve on the pen recorder graph. Richard Holmes sums up. |
| Master spool number: | 6HT/71452 |
| Production number: | 00525_1118 |
| Videofinder number: | 1832 |
| Available to public: | no |
