Available online
Description
This programme examines the structure of ribosomes and relates details of this structure to its functional role in protein synthesis.
This programme examines the structure of ribosomes and relates details of this structure to its functional role in protein synthesis.
| Module code and title: | S322, Biochemistry and molecular biology |
|---|---|
| Item code: | S322; 10; 1978 |
| First transmission date: | 30-06-1978 |
| Published: | 1978 |
| Rights Statement: | |
| Restrictions on use: | |
| Duration: | 00:24:00 |
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| Producers: | Roger Jones; Andrew Millington |
| Contributors: | Charles Kurland; Irene Ridge; George Stoffler |
| Publisher: | BBC Open University |
| Keyword(s): | DNA; Immuno-electron-microscopy; Messenger RNA; Protein synthesis; Ribosome; RNA; Transfer RNA |
| Footage description: | Animated diagram of a ribosome at work. Opening titles over. Charles Kurland introduces the programme. Using an animated diagram, Kurland explains how the genetic code in DNA is assembled, according to the Watts and Crick rules, into transfer RNA. Kurland next explains, with the aid of a graphics board, how the four ribo-nucleotides which constitute an amino acid are permuted to make up the various different amino acids. With the aid of animated diagrams, Kurland explains how the ribosome translates transfer RNA molecules in the protein synthesizing process. Kurland, using an animated diagram, discusses an experiment in which bacterial ribosomes wero split into their sub-units. Kurland goes on to explain how the composition of the two sub-units and their differences were determined by the electrophoresis. Animated diagrams help illustrate points made. Irene Ridge examines some electron micrographs of bacterial ribosomes in order to point out the gross structure of the sub-units. Ridge goes on to explain an experimental technique which allows some information on the internal structure of a ribosome to be determined. The technique maps the binding sites of antibodies on the surface of the ribosome. George Stoffler, who pioneered this method of determining the structure of ribosomes, begins to explain his experimental techniques. Electron micrographs and animated diagrams, with additional commentary by Irene Ridge, explain the method in more detail. Stoffler, with a large model of a ribosome, points out several of the antibody binding sites and explains what this tells us about the shape of the proteins in the ribosome. Charles Kurland explains what particular sections of the ribosome surface are doing during protein synthesis. He uses a large model of a ribosome to help illustrate his points. Kurland points out a problem which arises out of the conformation of the ribosome as now understood. It appears that there is insufficient room for the transfer RNA molecule to reach the interphase region of the ribosome. Kurland gives two possible solutions to this problem and then very briefly sums up the programme. |
| Master spool number: | 6HT/72735 |
| Production number: | 00525_1322 |
| Videofinder number: | 1017 |
| Available to public: | no |
