Available online
Description
Recently, a Unilever research team successfully used recombinant DNA techniques to transform a yeast host so that the host was consequently able to manufacture a foreign (plant) protein: Thaumatin.... This video-cassette uses the Unilever project as a case study to illustrate the procedures involved in such an undertaking. The main stages are: extract mRNA, synthesise cDNA, manufacture the appropriate recombinant plasmid, transform E.Coli, characterise the cDNA, transform the yeast host and harvest thaumatin. The cassette describes these stages by showing the laboratory techniques, interspersed with numerous animations. The viewer is invited, on nine separate occassions to stop the tape and tackle the questions in the video notes.
Recently, a Unilever research team successfully used recombinant DNA techniques to transform a yeast host so that the host was consequently able to manufacture a foreign (plant) protein: Thaumatin.... This video-cassette uses the Unilever project as a case study to illustrate the procedures involved in such an undertaking. The main stages are: extract mRNA, synthesise cDNA, manufacture the appropriate recombinant plasmid, transform E.Coli, characterise the cDNA, transform the yeast host and harvest thaumatin. The cassette describes these stages by showing the laboratory techniques, interspersed with numerous animations. The viewer is invited, on nine separate occassions to stop the tape and tackle the questions in the video notes.
| Module code and title: | S325, Biochemistry and cell biology |
|---|---|
| Item code: | S325; VC3 |
| First transmission date: | 1986 |
| Published: | 1986 |
| Rights Statement: | |
| Restrictions on use: | |
| Duration: | 01:00:00 |
| + Show more... | |
| Producer: | Jack Koumi |
| Contributor: | Hilary MacQueen |
| Publisher: | BBC Open University |
| Keyword(s): | E.Coli; Plasmid; RNA; Thaumatin; Unilever; Yeast |
| Master spool number: | HOU5078 |
| Production number: | FOUS364D |
| Videofinder number: | 1865 |
| Available to public: | no |
