High performance liquid chromatography

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Description
Separation of compounds through a column packed with silica in a solution know as the mobile phase.

Metadata describing this Open University video programme

Module code and title:	S344, Organic chemistry: a synthesis approach
Item code:	S344; VCR1
First transmission date:	1989
Published:	1989
Rights Statement:
Restrictions on use:
Duration:	00:25:30
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Producer:	Ted Smith
Contributor:	Roger Hill
Publisher:	BBC Open University
Keyword(s):	Chromatography liquid; Cycloisoleucine; Detector; Elute; Injector; Pump; Silica packed column
Subject terms:	High performance liquid chromatography
Footage description:	Footage - Thin layer chromatography (TLC). Chromatogram. Column chromatograph (0'02"-1'59") - Graphic of liquid chromatography (2'00"-4'01") - Gas chromatograph (4'02"-4'48") - Graphic of wavelengths (4'49"-5'35") - Gas chromatograph analysing dyes (5'36"-7'39") - Graphic of chromatogram and TLC (7'40"-9'00") - Graphic of hydrated silica separation. Normal and reverse phase of chloroform and silica. Graphic of biochemicals (9'26"-11'25") - Mixture of peptides separated using a gas chromatogram (12'03"-13'15") - Chromatogram. Analystical sepration of peptides (13'36"-15'20") - Isoleucine in refluxing ethane 1,2 diol. Graphic of cycloisoleucylisoleucine. Infrared spectrum of white solid (15'25"-17'45") - Graphic of stereoisomers structure (17'46"-18'00") - Flask of sodium ethoxide in ethanol, separation & TLC. Spectrum (18'01"-19'21") - HPLC. Chromatogram. Isomer peaks (19'22"-21'12") - Isomer mixture. C18 reverse phase column (21'13"-21'33"). Analytical chromatogram of silica and dichloromethane (22'00"-22'27") - Peaks of chromatogram (23'09-23'27") - Automatic sampler (23'35"-23'48") - Graphic of peaks and SS isomer of cycloisoleucylisoleucine (23'49"-24'55").
Master spool number:	HOU6196
Production number:	FOUS545S
Videofinder number:	2772
Available to public:	no