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Description
This programme is one of a series of six which concentrate on crucial biochemical techniques, then practice and applications, presented in a studio-based laboratory setting. After a brief introduct...ion by Professor Rose, Dr. Brian Tiplady demonstrates the principles and practice of polyacrylamide-gel electrophoresis. He demonstrates how this technique shows up the differences between the samples obtained in the purification of yeast hexokinase (see Programmes 1 and 2). Jeff Haywood demonstrates how the combination of electrophoresis and chromatography on paper can be used to show up the chemical differences between similar proteins. This technique is known as protein 'fingerprinting'. He uses as his examples haemoglobins - from normal human adults, from adults suffering from sickle cell disease, and from babies. Finally Dr. Tiplady uses a spectrophotometer to show how it is possible to monitor the uptake of oxygen by human blood. From such experiments it is possible to show that the previously demonstrated structural difference between the haemoglobin of a baby and its mothers is of functional importance while the baby is in the womb.
This programme is one of a series of six which concentrate on crucial biochemical techniques, then practice and applications, presented in a studio-based laboratory setting. After a brief introduct...ion by Professor Rose, Dr. Brian Tiplady demonstrates the principles and practice of polyacrylamide-gel electrophoresis. He demonstrates how this technique shows up the differences between the samples obtained in the purification of yeast hexokinase (see Programmes 1 and 2). Jeff Haywood demonstrates how the combination of electrophoresis and chromatography on paper can be used to show up the chemical differences between similar proteins. This technique is known as protein 'fingerprinting'. He uses as his examples haemoglobins - from normal human adults, from adults suffering from sickle cell disease, and from babies. Finally Dr. Tiplady uses a spectrophotometer to show how it is possible to monitor the uptake of oxygen by human blood. From such experiments it is possible to show that the previously demonstrated structural difference between the haemoglobin of a baby and its mothers is of functional importance while the baby is in the womb.
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