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This programme describes the techniques which were used to determine the structure of lysozyme and emphasises certain distinctive features of the molecule.
Metadata describing this Open University video programme
Module code and title: S322, Biochemistry and molecular biology
Item code: S322; 03
First transmission date: 31-03-1977
Published: 1977
Rights Statement:
Restrictions on use:
Duration: 00:23:45
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Producer: Roger Jones
Contributor: David Phillips
Publisher: BBC Open University
Keyword(s): 3-D structure; Computer animations; Lysozyme; Video animations
Footage description: Film, taken at 2000 magnifications, of bacterial cells being broken down by the enzyme lysozyme. Commentary by David Phillips. Shots of an atomic model of lysozyme showing its 129 amisso acid residues spread out in one long line. David Phillips walks alongside the model and points out some of the individual residues. Shots of a high resolution atomic model of lysozyme folded into its actual globular structure. Phillips explains how electron density maps are built up for a molecule like lysozyme by the technique of X-ray diffraction. Shots of the equipment with an explanation by Phillips of how it works. Shots of electron density maps. Phillips goes on to explain how it is possible to determine which contours on the electron density map represent which residues in the molecule. Electron density maps, atomic models and computer animations help illustrate his points. Phillips next demonstrates a more sophisticated method for fitting a molecule to an electron density map. This method, developed at Leeds University, uses a computer to calculate contours for a single value of election density for cross sections in three directions. Computer animations show the result. Commentary by Phillips. A computer animation builds up a 3 dimensional image of the lysozyme molecule. For clarity, side chains are not shown. Most of the residues are coloured red but those which make up the three helices are coloured green. Shots of a low resolution atomic model superimposed over a high resolution model of lysozyme in order that the structure may be seen more clearly. While Phillips sums up, shots of the atomic model of lysozyme viewed from above to reveal a distinct cleft in the globular shape.
Master spool number: 6HT/72182
Production number: 00525_1260
Videofinder number: 1009
Available to public: no