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This programme examines the increasing use of enzymes as industrial catalysts and concentrates on the medical importance of cholesterol oxidase, the main constituent of a diagnostic assay for blood... serum cholesterol.
Metadata describing this Open University video programme
Module code and title: S322, Biochemistry and molecular biology
Item code: S322; 07
First transmission date: 18-05-1977
Published: 1977
Rights Statement:
Restrictions on use:
Duration: 00:24:00
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Producer: Denis Gartside
Contributors: Peter Dunnill; Malcolm Lilly; Bill Richmond; Irene Ridge
Publisher: BBC Open University
Keyword(s): Atherosclerosis; Blood serum cholesterol; Clinical applications; Industrial enzyme extraction; Large scale production; Penicillin
Footage description: Irene Ridge introduces the programme with a very brief history of the industrial use of enzymes. Film of beer fermenting in a large vat. Still shot of newspaper clippings. Over film shots of their laboratory Malcolm Lilly and Peter Dunnill (University College, London) explain how they developed a system for obtaining enzymes on a large scale. Shots of the apparatus and animated diagrams of the process. Irene Ridge explains, briefly, how Lilly and Dunnill's technique was used to synthesise penicillin. Shots of apparatus and animated diagram. She then goes on to introduce the next sequence. Bill Richmond (Clinical Research Centre, Harrow) explains how he developed an enzyme based assay for blood serum cholesterol. He explains in some detail how the enzyme used in the assay could be extracted. Richmond goes on to demonstrate how the enzyme blood serum cholesterol test works. He then looks in some detail at the disease atherosclerosis. Several micrographs of aorta tissue, shots of affected arteries and a cross section of a normal and diseased aorta. Over diagrams of the ring structure of cholesterol, Richmond sums up the mechanism of the cholesterol screening test. Irene Ridge and Bill Richmond examine one of the properties of the enzyme cholesterol oxidase, the maximum reaction velocity. Shots of the experimental apparatus, a U-V spectrophotometer, several graphs, some animated, illustrate points made. Ridge discusses, briefly, the property of specificity of cholesterol oxidase. Using several diagrams, Malcolm Lilly examines, in some detail, the process by which cholesterol oxidase was produced in large quantities with a purity adequate for clinical use. Shots of apparatus as he talks. Lilly and Ridge discuss other important uses of the enzyme, particularly as a route to isolation of a wide range of steroids. Several diagrams of ring structures and film shots of contraceptive manufacture help illustrate points made. Lilly sums up the programme.
Master spool number: 6HT/72484
Production number: FOU1576P
Videofinder number: 1013
Available to public: no