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This programme is concerned with the ultrastrueture of the Golgi apparatus and with techniques used in research on milk production in conscious animals and in isolated mammary glands.
Metadata describing this Open University video programme
Module code and title: S321, Physiology of cells and organisms
Item code: S321; 05
First transmission date: 07-04-1974
Published: 1974
Rights Statement:
Restrictions on use:
Duration: 00:24:02
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Producer: Roger Jones
Contributors: Richard Holmes; J L Linzell
Publisher: BBC Open University
Keyword(s): Glucose; Golgi apparatus; Isolated mammary glands; Milk production; Mitosis; Primary cell plate; Protozoa; 'Scaly flagellates'; Ultrastructure
Footage description: Richard Holmes introduces the programme. He lists some of the functions of the Golgi apparatus. Holmes shows a magnified picture of a plant cell. He points out the cell components. Holmes them shows a simplified diagram of the same cell. He points out the membrane continuity throughout the cell. Film shots under magnification of the protozoan Trichonympha in the gut of a termite. Shots of a stained and mounted Trichonympha. Holmes points out the Golgi apparatus in the animal. Holmes discusses an experiment which demonstrates the relationship between the Golgi body and other membranes in the cell. The method is cell starvation and Holmes knows several shots of Trichonmypha taken during such an experiment. Effect on Golgi body and ER seen. Holmes discusses an experiment with amoeba which also demonstrates the relationship between Golgi and ER. The experiment involves enucleation and replacement of the cell nucleus. Still shots show the results. Holmes with a still shot (under magnification) of Englena (?) Golgi body. He points out out some of the characteristics of Golgi bodies. Holmes with a 3-D model of a Golgi body. He points out the charactersistic points as seen on micrographs. Film shots of mitosis in progress. The cell is between metaphase and telophase. Holmes explains the role of the Golgi apparatus in Mitosis. Holmes continues with a still shot of the cell during mitosis. Shots of electron micrograph of the Golgi body during mitosis. Micrographs show what appear to be vesicles produced by the Golgi body being transported to the cell plate during mitosis. Holmes explains the Carbon 14 pulse chase labelling method of studying the functions of the Golgi body in a dynamic system. Still shots taken during such an experiment shown. Holmes sums up the functions of the Golgi body. Electron micrographs of scales formed by the golgi body of a scaly flagellate. The intricate pattern of the scales is pointed out. Electron micrographs of a golgi body which is in the process of forming scales. Holmes introduces film sequences showing methods of obtaining physiological data on milk secretion. Film equence taken at the Agricultural Research Council Laboratary, Cambridge shows the mechanisms used to study milk secretion in ruminants J.L. Linzell explains. Shots of goats which have been surgically prepared for the experiment. Shots of the experiment in process. Commentary explains what is happening. Linzell discusses some of the difficulties encountered in developing this method. Farther shots of the experiment in progress. Diagram of an udder cell showing apical membrane and Golgi apparatus. Commentary disusses role of Golgi in milk production.
Master spool number: 6HT/71228
Production number: 00525_1109
Videofinder number: 1822
Available to public: no